Biomolecular NMR Facility

Department of Chemistry and Biochemistry

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C13_DEPT 2D for Structure Analysis Saturation Transfer Differenace (STD) Diffusion Ordered Spectroscopy(DOSY) RNA HSQC Protein Sequential Assignment Protein NOESY NMR Titration Parallel Stranded DNA Probe Sensitivity
NMR Sample Preparation Experiment Preparation 1D 1H 1D 31P 1D WaterGate 2D Experiment Setup 1H-15N HSQC 1H-13C HSQC 1H-15N SoFast-HMQC 2D TOCSY 2D NOESY Triple Resonance Experiments Saturation Transfer Difference NMR BBFO Probe Experiments Non-Uniform Sampling(NUS)
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13C DEPT: To differentiate different types of carbons in a chemical compound

C13DEPT

2D Experiments for Chemical Structural Characterization: Looking for connectivity

2D

Saturation Transfer Difference (STD): To identify biomolecule/ligand interactions

STD

Diffusion Ordered Spectroscopy (DOSY): To resolve different compounds in a mixture based on differing translational diffusion coefficients

DOSY

RNA HSQC experiments: Identifying secondary structure elements

RNA

Run triple resonance experiments to establish residue connectivity in a protein sequence

Sequential

NOESY Experiment: To identify proximate protons in space and in adjacent residues

NOESY

NMR Titration: To quantify a protein/ligand interaction, and more...

titration

NMR Evidence of DNA parallel-strand formation in solution

DNA
Comparison of probe sensitity

Probe sensitity